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KMID : 0368420220650030199
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Journal of Plant Biology
2022 Volume.65 No. 3 p.199 ~ p.216
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Characterization and Functional Explorations of O-glycosylation Enzymes SECRET AGENT and SPINDLY in Pyrus bretschneideri
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Gao Hongru
Tan Qiuping
Li Qionghou
Chen Guodong
Tang Chao
Cao Peng
Qian Ming
Zhang Mingliang
Wang Peng
Zhang Shaoling
Wu Juyou
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Abstract
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O-linked β-N-acetylglucosamine (O-GlcNAc) modification, a specific and ubiquitous post-translational modification of proteins, has been verified to play an irreplaceable role in diverse biological processes. O-GlcNAc transferases (OGTs) catalyze the addition of N-acetylglucosamine (GlcNAc) to proteins. Former studies had classified OGTs in Arabidopsis into two categories: SECRET AGENT (SEC) and SPINDLY (SPY), as they were both characterized to possess an OGT catalytic domain in C-terminal and a protein interaction domain (tetratricopeptide repeats) in N-terminal. On account of the unexplored blanks that remain in the O-glycosylation enzymes SEC and SPY of the Rosaceae species, we tend to determine the complete list of members for SEC and SPY gene families in eight Rosaceae species, and explore the potential biological functions as well as their correlative molecular mechanisms for SEC and SPY of pear (Pyrus bretschneideri) specifically in this study. The whole members of SECs and SPYs in pear, as well as their protein structures, chromosomal distributions, evolutionary profiles, expression patterns and subcellular localizations are identified. Through stable overexpression and mutant complementation assays, we verify the positive-regulation role of PbrSEC1 in the plant growth and development. The protein interaction involved is ascertained by different assays. Moreover, expression inhibition of PbrSPY1 via anti-sense oligodeoxynucleotides (as-ODN) indicates that PbrSPY1 may have a potential function in the process of pear pollen tube growth. Our study reveals member identification and evolution profiles of SEC and SPY gene families in eight Rosaceae species, specifically along with expression pattern, subcellular localization, biological functions and relative potential molecular mechanisms of PbrSECs and PbrSPYs.
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KEYWORD
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O-glycosylation enzymes, O-GlcNAc transferase, SECRET AGENT, SPINDLY, Pear (Pyrus bretschneideri), Rosaceae, Plant growth, Pollen tube growth
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